Translational_Unit

Part:BBa_K1679019

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-07)

GFP+Promoter+RNA thermometer (FourU) +mRFP

This is a Translational_Unit containing, a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040), two terminator(BBa_B0010 and BBa_B0012), a promoter(BBa_J23119K),a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.

This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device. It's a intermediate before we constructing BBa_K1679030

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 890
    Illegal NheI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1539
    Illegal AgeI site found at 1651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662
[edit]
Categories
Parameters
None